An in-vivo experimental evaluation of the efficacy of fish-derived antimicrobial peptides against multidrug-resistant Pseudomonas aeruginosa

Introduction: due to the fact that antimicrobial
peptides antimicrobial peptides (AMPs) from
climbing perch have not been fully explored for
their antimicrobial potency, this investigation was
undertaken to explore that possibility.
Methods: antimicrobial peptides (AMPs) from the
mucous secretion of climbing perch were
obtained and an in-vivo analysis was conducted
using mice. Results: the results showed inhibitory
effects on multidrug-resistant multidrug-resistant
Pseudomonas aeruginosa with reduced mortality
from 100% among the non-treated group to 25%.
Similarly, the level of serum transaminase enzymes
(AST and ALT), creatinine levels, and proinflammatory cytokines (TNF-α and IL-6) were all
found to be higher in the non-treatment group
compared to the AMP-treatment group. Also,
extensive tissue damage in the lung, liver, and
spleen of the non-treated control group mice was
observed based on the histopathological lesions
recorded. As expected, AMPs from climbing perch
significantly alleviated multidrug-resistant P.
aeruginosa infection in-vivo and produced
enhanced therapeutic efficacy superior to the
ciprofloxacin treatment. Conclusion: this study
provides insight into the potential antimicrobial
activity of fish innate immune system-derived
peptides that could serve as a candidate for the
substitute of antibiotics