Introduction: Somalia is among the countries with a high burden of human papillomavirus (HPV) infection in Sub-Saharan Africa. In 2022,
660,000 infections are reported among women globally, out of which 350,000 died from the disease. Most of the studies on HPV reported from
Somalia are based on cytologic analysis which is a subjective and suboptimal assessment. The recent World Health Organization (WHO)
guidelines for HPV recommend DNA-based testing as a better alternative to the traditional pap smear test.
Methodology: This study was undertaken to determine the prevalence of HPV based on the preferred HPV DNA assay on cervical samples of
women of reproductive age in Mogadishu, Somalia. The HPV DNA detection was carried out using real-time quantitative polymerase chain
reaction (RT-PCR) assays to identify high-risk oncogenic HPV16, HPV18, and other high-risk HPV types.
Results: Overall, 31.7% (60/189) of cervical samples were positive for HPV DNA. Out of this, 19.6% were high risk-HPV (hrHPV), 13.8%
were HPV16, and 5.3% were HPV18. Moreover, age, income and education level were found to be significant risk factors for HPV infection.
Conclusions: These results provide further proof that HPV continues to be a serious public health challenge in Somalia with the risk of
progressing to fatal cervical cancer.
Key words: human papillomavirus; cervical cancer; risk factors; HPV-DNA; Somalia.